An exocytotic mutant of Paramecium caudatum: membrane fusion without secretory contents release
نویسندگان
چکیده
منابع مشابه
An exocytotic mutant of ParalJ 1 eciulJ 1 caudatulJ 1 : membrane fusion without secretory contents release
This is a detailed characterization of a secretory mutant incapable of releasing secretory contents despite normal exocytotic membrane fusion performance. Trichocyst non-discharge strain tnd1 of Paramecium caudatum and its wildtype (wt) both show a transient cortical [Ca+l; increase and exocytotic membrane fusion in response to the polyamine secretagogue, aminoethyldextran (AEO), or to caffeine...
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Work with Paramecium has contributed to the actual understanding of certain aspects of exocytosis regulation, including membrane fusion. The system is faster and more synchronous than any other dense-core vesicle system described and its highly regular design facilitates correlation of functional and ultrastructural (freeze-fracture) features. From early times on, several crucial aspects of exo...
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Membrane vesicles with a high mating reactivity were obtained from cilia of Paramecium caudatum by treatment with a solution containing 2 M urea and 0.1 mM Na2-EDTA. All processes of conjugation were induced in cells of the complementary mating type by approximately 10 mug/ml proteins of the vesicles. Electron microscope observation showed that the membrane vesicles have a diameter of 100-150 n...
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ژورنال
عنوان ژورنال: Cell Calcium
سال: 1998
ISSN: 0143-4160
DOI: 10.1016/s0143-4160(98)90030-6